Tag: Biofilm

Harbor MedTech Presents the Latest Findings on its Advanced Wound-Healing

     Technology at the Symposium on Advanced Wound Care and Wound Healing Society Spring Conference

 

Harbor MedTech, a commercial-stage regenerative medicine company delivering innovative biologic wound-healing products, presented a Poster on Architect®, its advanced wound-healing product, and BriDGE®, its unique technology.

 

The scientific poster, “Stabilized Collagen Matrix Dressing Improves Macrophage Recruitment and Wound Epithelialization” was presented at the Symposium on Advanced Wound Care and Wound Healing Society Spring Conference, April 5 – April 9, 2017, in San Diego, California and was selected for the WHS Industrial Research & Development Poster Award Competition.

 

The poster was a presentation of ongoing research, led by Dr. Chandan K. Sen, Executive Director of Ohio State University’s Comprehensive Wound Center. Dr. Sen’s work involves a variety of in vitro and animal studies that initially describe the mechanism of action of Architect, a stabilized collagen matrix, for the treatment of a variety of wound types. These studies suggest that Architect may serve as a scaffold for cells within the wound microenvironment and may provide effective defenses against bacterial colonization and wound infection. In vivo, application of Architect stimulated … read more

Oxygen therapies for wound healing: EWMA findings and recommendations

For wounds to heal, it is essential that macro- and microcirculation is restored in the surrounding tissue (Niinikoski et al, 1991; Gottrup, 2004a). One of the most urgent requirements is oxygen, as it is critically important for the reconstruction of new vessels and connective tissue, and also enables resistance to infection … View PDF

The International Wound Infection Institute (IWII) is an organisation of
volunteer interdisciplinary health professionals dedicated to advancing
and improving practice relating to prevention and control of wound
infection. This includes acute wounds (surgical, traumatic and burns)
and chronic wounds of all types, although principally chronic wounds of
venous, arterial, diabetic and pressure aetiologies.

 

Wound infection is a common complication of wounds. It leads to delays in wound
healing and increases the risk of loss of limb and life. Implementation of effective
strategies to prevent, diagnose and manage, is important in reducing mortality and
morbidity rates associated with wound infection.

 

This second edition of Wound Infection in Clinical Practice is an update of the first edition
published in 2008 by the World Union of Wound Healing Societies (WUWHS). The
original document was authored by leading experts in wound management and endorsed
by the WUWHS. The intent of this edition is to provide a practical, updated resource that
is easy-to-use and understand.

read more (pdf)

Combating Biofilms In The Chronic Wound

Given the complexity of biofilm in lower extremity wounds, these authors offer a closer look on how biofilm develops, keys to eradicating biofilm and emerging modalities that may have an impact in the future.

 

We all encounter biofilms on a regular basis in our practices. A biofilm is a complex polymicrobal community of bacteria and fungi that develops on foreign materials, necrotic debris, exposed bone, and within the bed of chronic wounds. When James and colleagues examined the biopsies of 50 chronic wound beds, 60 percent contained a biofilm.

 

Planktonic or free-floating bacteria are more aggressive and divide more rapidly. Changes in gene expression allow them to secrete hydrolase enzymes and exotoxins, resulting in more rapid local tissue invasion. As a bacterial colony develops, environmental stimuli induce the cells to engage in quorum sensing, a gradient-based recruitment strategy used to summon additional bacteria to the developing biofilm and alter the phenotypic expression of bacteria within the community. Free-floating planktonic bacteria adhere to the wound bed using very weak molecular interactions … read more

Biofilms and Inflammation in Chronic Wounds

Abstract

Significance

The incidence, cost, morbidity, and mortality associated with non-healing of chronic skin wounds are dramatic. With the increasing numbers of people with obesity, chronic medical conditions, and an increasing life expectancy, the healthcare cost of non-healing ulcers has recently been estimated at $25 billion annually in the United States. The role played by bacterial biofilm in chronic wounds has been emphasized in recent years, particularly in the context of the prolongation of the inflammatory phase of repair.

Recent Advances

Rapid high-throughput genomic approaches have revolutionized the ability to identify and quantify microbial organisms from wounds. Defining bacterial genomes and using genetic approaches to knock out specific bacterial functions, then studying bacterial survival on cutaneous wounds is a promising strategy for understanding which genes are essential for pathogenicity.

Critical Issues

When an animal sustains a cutaneous wound, understanding mechanisms involved in adaptations by bacteria and adaptations by the host in the struggle for survival is central to development of interventions that favor the host.

Future Directions

Characterization of microbiomes of clinically well characterized chronic human wounds is now under way. The use of in vivo models of biofilm-infected cutaneous wounds will permit the study of the mechanisms needed for biofilm formation, persistence, and potential synergistic interactions among bacteria. A more complete understanding of bacterial survival mechanisms and how microbes influence host repair mechanisms are likely to provide targets for chronic wound therapy.

full article

TIME to face the challenge of wound infection

Complex wounds, Events, Infection | Schulke
TIME to face the challenge of wound infection.
This microsite follows on from the plenary session,
chaired by Kath Vowden at the Wounds UK conference, Harrogate 2016.

Click here to view the microsite

What Are Bacterial Biofilms? A Six Minute Montage

This is a six-minute video clip: a four minute montage of clips from early interviews with biofilm researchers, then a 3D animation showing the “life cycle” of bacterial biofilm — including its dispersal into the bloodstream.

 

Biofilm and Wound Healing

Composition of Biofilm

Biofilm is a complex microbial community containing self- and surface-attached microorganisms that are embedded in an extracellular polymeric substance.1,2 The extracellular polymeric substance is a primarily polysaccharide protective matrix synthesized and secreted by the microorganisms that attaches the biofilm firmly to a living or non-living surface. This protective covering does not allow the body’s immune system to recognize the presence of the microorganism; therefore, the bacteria evade an immune response, avoid detection by standard diagnostic techniques, and avoid destruction by standard treatments. Because of the tenacity of the attached biofilm, the microoganisms are able to resist physical forces, such shear, and are able to withstand nutrient and moisture deprivation, altered pH, and the impact of antibiotics and antiseptics. For the purposes of this discussion we break down the formation and actions of biofilms and discuss their impact on wound healing … read more