Bioactive Composite Nanofiber Mat: Clematis hirsuta for Regenerative Wound Healing
Summary: This study introduces a novel electrospun nanofibrous composite mat blending polyvinylpyrrolidone/polyvinyl alcohol (PVP/PVA) with White Clematis hirsuta (CH) extract to tackle acute and chronic wound challenges, especially in low-resource settings. GC-MS identified key bioactives like phenolic acids and squalene for anti-inflammatory/antibacterial properties. The mat’s uniform fibers (246–428 nm) improved hydrophilicity, water vapor transmission (up to 2178 g/m²/day), and sustained release (68% over 72h), with 3% CH boosting E. coli/S. aureus inhibition (21.5–21.8 mm zones). Biocompatible (117% fibroblast viability), it accelerated in vivo healing to 95.2% closure via angiogenesis, collagen deposition, and reduced inflammation, offering a sustainable, non-cytotoxic alternative to silver dressings.
Key Highlights:
- CH integration via electrospinning yielded bead-free nanofibers with confirmed bioactive loading (FTIR/XRD) and morphology (SEM).
- Antibacterial efficacy: 3% CH mats inhibited E. coli (21.5 mm) and S. aureus (21.8 mm), outperforming controls.
- Physicochemical: WVTR rose to 2178 g/m²/day; biodegradation to 17.7% (vs. 11.4% control); 68% release over 72h.
- Biocompatibility: Enhanced fibroblast viability (114–117% at 2–3% CH); no cytotoxicity.
- In vivo: 95.2% wound closure, boosted re-epithelialization/collagen, and angiogenesis; supports SDG 3 for accessible care.
Keywords: Clematis hirsuta, nanofiber mat, regenerative wound healing, antibacterial dressing, sustainable wound care